Background:

This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.

Applications:

IF

Name of antibody:

MDM2(Phospho-Ser186/Ser188)

Immunogen:

Peptide sequence around phosphorylation site of Ser186/Ser188(H-K-S(p)-D-S(p)-I-S) derived from Human MDM2.

Full name:

MDM2 proto-oncogene, E3 ubiquitin protein ligase

Synonyms：

HDMX; hdm2; ACTFS

SwissProt:

Q00987

IF positive control:

Hela cells

IF Recommend dilution:

100-200